2',3'-Cyclic-nucleotide 3'-phosphodiesterase
| 2',3'-Cyclic-nucleotide 3'-phosphodiesterase | |||||||
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| Identifiers | |||||||
| EC number | 3.1.4.37 | ||||||
| CAS number | 60098-35-3 | ||||||
| Databases | |||||||
| IntEnz | IntEnz view | ||||||
| BRENDA | BRENDA entry | ||||||
| ExPASy | NiceZyme view | ||||||
| KEGG | KEGG entry | ||||||
| MetaCyc | metabolic pathway | ||||||
| PRIAM | profile | ||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||
| Gene Ontology | AmiGO / EGO | ||||||
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2',3'-Cyclic-nucleotide 3'-phosphodiesterase also known as CNPase is an enzyme that in humans is encoded by the CNP gene.[1][2]
Contents |
Reaction
CNPase catalyzes the following reaction:
- nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 2'-phosphate
Thus, the two substrates of this enzyme are nucleoside 2',3'-cyclic phosphate and H2O, whereas its product is nucleoside 2'-phosphate.
Function
CNPase is a myelin-associated enzyme that makes up 4% of total CNS myelin protein, and is thought to undergo significant age-associated changes.[3] It is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides, though a cohesive understanding of its specific physiologic functions are still ambiguous.[4]
Structural studies have revealed that four classes of CNPs belong to one protein superfamily. CNP's catalytic core consists of three alpha-helices and nine beta-strands. The proposed mechanism of CNPs phosphodiesterase catalytic activity is similar to the second step of the reaction mechanism for RNase A.[5]
CNP is expressed exclusively by oligodendrocytes in the CNS, and the appearance of CNP seems to be one of the earliest events of oligodendrocyte differentiation.[6] CNP is thought to play a critical role in the events leading up to myelination.[7]
CNP also associates with microtubules in brain tissue and FRTL-5 thyroid cells, and is reported to have microtubule-associated protein-like activity (MAP; see MAP2), being able to catalyze microtubule formation at low molar ratios. Deletion of the C-terminus of CNP or phosphorylation abolish the catalytic activity of microtubule formation. CNP can link tubulin to cellular membranes, and might be involved in the regulation cytoplasmic microtubule distribution.[8]
References
- ^ Sprinkle TJ, Lanclos KD, Lapp DF (Aug 1992). "Assignment of the human 2',3'-cyclic nucleotide 3'-phosphohydrolase gene to chromosome 17". Genomics 13 (3): 877–80. doi:10.1016/0888-7543(92)90174-Q. PMID 1322358.
- ^ "Entrez Gene: CNP 2',3'-cyclic nucleotide 3' phosphodiesterase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1267.
- ^ Hinman JD, Chen CD, Oh SY, Hollander W, Abraham CR (January 2008). "Age-dependent accumulation of ubiquitinated 2',3'-cyclic nucleotide 3'-phosphodiesterase in myelin lipid rafts". Glia 56 (1): 118–33. doi:10.1002/glia.20595. PMID 17963267.
- ^ Kursula P (February 2008). "Structural properties of proteins specific to the myelin sheath". Amino Acids 34 (2): 175–85. doi:10.1007/s00726-006-0479-7. PMID 17177074.
- ^ Sakamoto Y, Tanaka N, Ichimiya T, Kurihara T, Nakamura KT (February 2005). "Crystal structure of the catalytic fragment of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase". J. Mol. Biol. 346 (3): 789–800. doi:10.1016/j.jmb.2004.12.024. PMID 15713463.
- ^ Kasama-Yoshida H, Tohyama Y, Kurihara T, Sakuma M, Kojima H, Tamai Y (October 1997). "A comparative study of 2',3'-cyclic-nucleotide 3'-phosphodiesterase in vertebrates: cDNA cloning and amino acid sequences for chicken and bullfrog enzymes". J. Neurochem. 69 (4): 1335–42. doi:10.1046/j.1471-4159.1997.69041335.x. PMID 9326261.
- ^ Gravel M, Peterson J, Yong VW, Kottis V, Trapp B, Braun PE (June 1996). "Overexpression of 2',3'-cyclic nucleotide 3'-phosphodiesterase in transgenic mice alters oligodendrocyte development and produces aberrant myelination". Mol. Cell. Neurosci. 7 (6): 453–66. doi:10.1006/mcne.1996.0033. PMID 8875429.
- ^ Bifulco M, Laezza C, Stingo S, Wolff J (February 2002). "2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin". Proc. Natl. Acad. Sci. U.S.A. 99 (4): 1807–12. doi:10.1073/pnas.042678799. PMC 122275. PMID 11842207. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=122275.
Further reading
- Drummond GI, Iyer NT and Keith J (1962). "Hydrolysis of ribonucleoside 2',3'-cyclic phosphates by a diesterase from brain". J. Biol. Chem. 237: 3535–3539.
- Helfman DM, Kuo JF (1982). "A homogeneous cyclic CMP phosphodiesterase hydrolyzes both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides". J. Biol. Chem. 257 (2): 1044–7. PMID 6274851.
- Helfman DM, Shoji M, Kuo JF (1981). "Purification to homogeneity and general properties of a novel phosphodiesterase hydrolyzing cyclic CMP and cyclic AMP". J. Biol. Chem. 256 (12): 6327–34. PMID 6263914.
- Kurihara T, Nishizawa Y, Takahashi Y, Odani S (1981). "Chemical, immunological and catalytic properties of 2':3'-cyclic nucleotide 3'-phosphodiesterase purified from brain white matter". Biochem. J. 195 (1): 153–7. PMC 1162865. PMID 6272743. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1162865.
- Nishizawa Y, Kurihara T, Takahashi Y (1980). "Spectrophotometric assay, solubilization and purification of brain 2':3'-cyclic nucleotide 3'-phosphodiesterase". Biochem. J. 191 (1): 71–82. PMC 1162183. PMID 6258586. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1162183.
- Thompson RJ (1992). "2',3'-cyclic nucleotide-3'-phosphohydrolase and signal transduction in central nervous system myelin.". Biochem. Soc. Trans. 20 (3): 621–6. PMID 1385234.
- Leroy MJ, Dumler I, Lugnier C, et al. (1992). "A new peptide (1150Da) selectively activates the calcium-calmodulin sensitive isoform of cyclic nucleotide phosphodiesterase from human myometrium.". Biochem. Biophys. Res. Commun. 184 (2): 700–5. doi:10.1016/0006-291X(92)90646-3. PMID 1315529.
- Douglas AJ, Fox MF, Abbott CM, et al. (1992). "Structure and chromosomal localization of the human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene.". Ann. Hum. Genet. 56 (Pt 3): 243–54. doi:10.1111/j.1469-1809.1992.tb01149.x. PMID 1360194.
- Staugaitis SM, Bernier L, Smith PR, Colman DR (1990). "Expression of the oligodendrocyte marker 2'3'-cyclic nucleotide 3'-phosphodiesterase in non-glial cells.". J. Neurosci. Res. 25 (4): 556–60. doi:10.1002/jnr.490250413. PMID 2161933.
- Agrawal HC, Sprinkle TJ, Agrawal D (1990). "2',3'-cyclic nucleotide-3'-phosphodiesterase in the central nervous system is fatty-acylated by thioester linkage.". J. Biol. Chem. 265 (20): 11849–53. PMID 2164018.
- Kurihara T, Takahashi Y, Nishiyama A, Kumanishi T (1988). "cDNA cloning and amino acid sequence of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase.". Biochem. Biophys. Res. Commun. 152 (2): 837–42. doi:10.1016/S0006-291X(88)80114-1. PMID 2835044.
- Sprinkle TJ, McMorris FA, Yoshino J, DeVries GH (1985). "Differential expression of 2':3'-cyclic nucleotide 3'-phosphodiesterase in cultured central, peripheral, and extraneural cells.". Neurochem. Res. 10 (7): 919–31. doi:10.1007/BF00964629. PMID 2995854.
- Sheedlo HJ, Doran JE, Sprinkle TJ (1984). "An investigation of 2':3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37, CNP) in peripheral blood elements and CNS myelin.". Life Sci. 34 (18): 1731–7. doi:10.1016/0024-3205(84)90572-1. PMID 6328143.
- Monoh K, Kurihara T, Takahashi Y, et al. (1993). "Structure, expression and chromosomal localization of the gene encoding human 2',3'-cyclic-nucleotide 3'-phosphodiesterase.". Gene 129 (2): 297–301. doi:10.1016/0378-1119(93)90283-9. PMID 8392017.
- Löbbert RW, Winterpacht A, Seipel B, Zabel BU (1997). "Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A)--a gene involved in fat metabolism located at 11p 15.1.". Genomics 37 (2): 211–8. doi:10.1006/geno.1996.0544. PMID 8921398.
- Stricker R, Kalbacher H, Reiser G (1997). "The epitope recognized by a monoclonal antibody in the myelin-associated protein CNP.". Biochem. Biophys. Res. Commun. 237 (2): 266–70. doi:10.1006/bbrc.1997.7125. PMID 9268698.
- O'Neill RC, Braun PE (2000). "Selective synthesis of 2',3'-cyclic nucleotide 3'-phosphodiesterase isoform 2 and identification of specifically phosphorylated serine residues.". J. Neurochem. 74 (2): 540–6. doi:10.1046/j.1471-4159.2000.740540.x. PMID 10646504.
- Zauli G, Milani D, Mirandola P, et al. (2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway.". FASEB J. 15 (2): 483–91. doi:10.1096/fj.00-0354com. PMID 11156964.
- Bifulco M, Laezza C, Stingo S, Wolff J (2002). "2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin.". Proc. Natl. Acad. Sci. U.S.A. 99 (4): 1807–12. doi:10.1073/pnas.042678799. PMC 122275. PMID 11842207. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=122275.
- Davidoff MS, Middendorff R, Köfüncü E, et al. (2002). "Leydig cells of the human testis possess astrocyte and oligodendrocyte marker molecules.". Acta Histochem. 104 (1): 39–49. doi:10.1078/0065-1281-00630. PMID 11993850.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
- Basrur V, Yang F, Kushimoto T, et al. (2003). "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.". J. Proteome Res. 2 (1): 69–79. doi:10.1021/pr025562r. PMID 12643545.
- Kozlov G, Lee J, Elias D, et al. (2003). "Structural evidence that brain cyclic nucleotide phosphodiesterase is a member of the 2H phosphodiesterase superfamily.". J. Biol. Chem. 278 (46): 46021–8. doi:10.1074/jbc.M305176200. PMID 12947117.
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